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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages
doi: 10.1186/s13287-020-1561-x
Figure Lengend Snippet: PGE2 mediates the polarization effects of UCMSC-CM on macrophages. LPS-treated macrophages were stimulated with NS-398-CM, a mixture of UCMSC-CM with an anti-PGE2 neutralizing antibody, or NCM plus PGE2. Several cytokines derived from macrophages were detected by ELISA. a TNF-α. b IL-6. c IL-10. d VEGF. n = 6 per group. * P < 0.05, ** P < 0.01
Article Snippet: To determine the involvement of UCMSC-secreted PGE2 in UCMSC-CM-induced macrophage polarization, LPS-treated macrophages were stimulated with NS-398-CM or a mixture of UCMSC-CM with an
Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay
Journal: Stem Cell Research & Therapy
Article Title: Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages
doi: 10.1186/s13287-020-1561-x
Figure Lengend Snippet: PGE2 plays a role in the UCMSC-CM-induced functional recovery of diabetic vascular endothelial cells via remodeling of the macrophage phenotypes. High-glucose-induced HUVECs, which were indirectly cocultured with macrophages, were treated with NS-398-CM, a mixture of UCMSC-CM with an anti-PGE2 neutralizing antibody, or NCM plus PGE2. The functions of HUVECs were determined by angiogenesis assays, migration assays, and chemotaxis assays. a Representative images of the angiogenesis assay. Scale bar 200 μm. b Quantitative analysis of the total tube length in the angiogenesis assay. c Representative images of the migration assay. Scale bar 200 μm. d Quantitative analysis of the wound area in the migration assay. e Quantitative analysis of the chemotactic cell number in the chemotaxis assay. n = 6 per group. * P < 0.05, ** P < 0.01
Article Snippet: To determine the involvement of UCMSC-secreted PGE2 in UCMSC-CM-induced macrophage polarization, LPS-treated macrophages were stimulated with NS-398-CM or a mixture of UCMSC-CM with an
Techniques: Functional Assay, Migration, Chemotaxis Assay, Angiogenesis Assay
Journal: Stem Cell Research & Therapy
Article Title: Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages
doi: 10.1186/s13287-020-1561-x
Figure Lengend Snippet: Schematic model of UCMSCs promoting wound healing in diabetic mice. UCMSCs accelerate wound healing and improve angiogenesis in diabetic mice via the remodeling of the macrophage phenotypes. UCMSC-secreted PGE2 plays a crucial role in improving the restoration of vascular endothelial cell dysfunction via the remodeling of macrophage phenotypes, which subsequently improve the local microenvironment of vascular endothelial cells through the secretion of IL-10 and VEGF
Article Snippet: To determine the involvement of UCMSC-secreted PGE2 in UCMSC-CM-induced macrophage polarization, LPS-treated macrophages were stimulated with NS-398-CM or a mixture of UCMSC-CM with an
Techniques:
Journal: Stem Cell Research & Therapy
Article Title: Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages
doi: 10.1186/s13287-020-1561-x
Figure Lengend Snippet: PGE2 mediates the polarization effects of UCMSC-CM on macrophages. LPS-treated macrophages were stimulated with NS-398-CM, a mixture of UCMSC-CM with an anti-PGE2 neutralizing antibody, or NCM plus PGE2. Several cytokines derived from macrophages were detected by ELISA. a TNF-α. b IL-6. c IL-10. d VEGF. n = 6 per group. * P < 0.05, ** P < 0.01
Article Snippet: To determine the involvement of UCMSC-secreted
Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay
Journal: Stem Cell Research & Therapy
Article Title: Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages
doi: 10.1186/s13287-020-1561-x
Figure Lengend Snippet: PGE2 plays a role in the UCMSC-CM-induced functional recovery of diabetic vascular endothelial cells via remodeling of the macrophage phenotypes. High-glucose-induced HUVECs, which were indirectly cocultured with macrophages, were treated with NS-398-CM, a mixture of UCMSC-CM with an anti-PGE2 neutralizing antibody, or NCM plus PGE2. The functions of HUVECs were determined by angiogenesis assays, migration assays, and chemotaxis assays. a Representative images of the angiogenesis assay. Scale bar 200 μm. b Quantitative analysis of the total tube length in the angiogenesis assay. c Representative images of the migration assay. Scale bar 200 μm. d Quantitative analysis of the wound area in the migration assay. e Quantitative analysis of the chemotactic cell number in the chemotaxis assay. n = 6 per group. * P < 0.05, ** P < 0.01
Article Snippet: To determine the involvement of UCMSC-secreted
Techniques: Functional Assay, Migration, Chemotaxis Assay, Angiogenesis Assay
Journal: Stem Cell Research & Therapy
Article Title: Umbilical cord-matrix stem cells induce the functional restoration of vascular endothelial cells and enhance skin wound healing in diabetic mice via the polarized macrophages
doi: 10.1186/s13287-020-1561-x
Figure Lengend Snippet: Schematic model of UCMSCs promoting wound healing in diabetic mice. UCMSCs accelerate wound healing and improve angiogenesis in diabetic mice via the remodeling of the macrophage phenotypes. UCMSC-secreted PGE2 plays a crucial role in improving the restoration of vascular endothelial cell dysfunction via the remodeling of macrophage phenotypes, which subsequently improve the local microenvironment of vascular endothelial cells through the secretion of IL-10 and VEGF
Article Snippet: To determine the involvement of UCMSC-secreted
Techniques: